The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells.
IHC-P: Use at an assay dependent dilution (PMID 19720836).
IHC-Fr: 1/25 - 1/100.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myeloid Precursor Cells antibody [ER-MP58] (ab63860)Image from Lu X et al, J Biol Chem. 2009 Oct 16;284(42):29087-96. Epub 2009 Aug 31, Fig 2. DOI 10.1074/jbc.M109.035899
ab63860 staining Myeloid Precursor Cells in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunohistochemical analysis was performed with heat-induced antigen retrieval. The primary antibody used was ab63860. A biotinylated secondary antibody was used with and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin. Scale bar, 200 µm.
Karpurapu M et al. Functional PU.1 in macrophages has a pivotal role in NF-?B activation and neutrophilic lung inflammation during endotoxemia. Blood118:5255-66 (2011).
Read more (PubMed: 21937699) »
Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem284:29087-96 (2009).
Read more (PubMed: 19720836) »