Anti-Myeloid leukemia factor 1 抗体 (ab70211)

製品の概要

  • 製品名Anti-Myeloid leukemia factor 1 antibody
    Myeloid leukemia factor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myeloid leukemia factor 1
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residue 200 and the C-terminus (residue 256) of human Myeloid leukemia factor 1.

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
    • 組織特異性Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
    • 関連疾患Note=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
    • 配列類似性Belongs to the MLF family.
    • 翻訳後修飾Phosphorylation is required for binding to YWHAZ.
    • 細胞内局在Cytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Hls7 antibody
      • MLF1 antibody
      • MLF1_HUMAN antibody
      • Myelodysplasia myeloid leukemia factor 1 antibody
      • Myelodysplasia-myeloid leukemia factor 1 antibody
      • Myeloid leukemia factor 1 antibody
      • myeloid leukemia factor 1 variant 1 antibody
      • myeloid leukemia factor 1 variant 2 antibody
      • myeloid leukemia factor 1 variant 3 antibody
      see all

    Anti-Myeloid leukemia factor 1 antibody 画像

    • All lanes : Anti-Myeloid leukemia factor 1 antibody (ab70211) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg


      Predicted band size : 31 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,250 kDa,28 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human Myeloid leukemia factor 1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded). Affinity purified rabbit anti-Myeloid leukemia factor 1 antibody (ab70211) was used at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for WB detection. Lane 2 represents IP IgG control.

    Anti-Myeloid leukemia factor 1 antibody (ab70211) 使用論文

    ab70211 has not yet been referenced specifically in any publications.

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