製品の概要
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製品名Anti-Myelin PLP antibody
Myelin PLP 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Myelin PLP
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
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免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (IYGTASFFFL YGALLLAEGF YTTGAVRQIF GDYKTTICGK GLSATVTGGQ) of Human Myelin PLP (NP_000524)
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ポジティブ･コントロール
- HeLa cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab83032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
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関連疾患Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
配列類似性Belongs to the myelin proteolipid protein family.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 281410 Cow
- Entrez Gene: 481002 Dog
- Entrez Gene: 5354 Human
- Entrez Gene: 18823 Mouse
- Entrez Gene: 24943 Rat
- Omim: 300401 Human
- SwissProt: P04116 Cow
- SwissProt: P23294 Dog
see all -
別名
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
画像
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Western blot - Anti-Myelin PLP antibody (ab83032)Anti-Myelin PLP antibody (ab83032) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa (why is the actual band size different from the predicted?)
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
プロトコール
参考文献
ab83032 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"