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RabMAb

Anti-Myelin oligodendrocyte glycoprotein 抗体 [EP4281] (ab109746)

製品の概要

  • 製品名
    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]
    Myelin oligodendrocyte glycoprotein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the extracellular domain of Human Myelin oligodendrocyte glycoprotein

  • ポジティブ・コントロール
    • Mouse brain, Rat brain and Human brain lysates; Human astrocytoma tissue and Human brain tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:
    Anti-Myelin oligodendrocyte glycoprotein antibody (HRP) [EP4281] (ab199236)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
    • 組織特異性
      Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
    • 配列類似性
      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BTN6 antibody
      • BTNL11 antibody
      • MGC26137 antibody
      • MOG alpha 5 antibody
      • MOG alpha 6 antibody
      • MOG AluA antibody
      • MOG AluB antibody
      • MOG antibody
      • MOG Ig AluB antibody
      • MOG_HUMAN antibody
      • MOGIG2 antibody
      • Myelin oligodendrocyte glycoprotein antibody
      • Myelin-oligodendrocyte glycoprotein antibody
      • NRCLP7 antibody
      see all

    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] 画像

    • ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.
    • ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Human brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa
    • ab109746 showing negative staining in Breast carcinoma tissue.

    • ab109746 showing negative staining in Normal breast tissue.

    • ab109746 showing positive staining in Glioblastoma tissue.

    • ab109746 showing negative staining in Colonic adenocarcinoma tissue.

    • ab109746 showing negative staining in Normal tonsil tissue.

    • ab109746 showing negative staining in Normal colon tissue.

    • ab109746 showing negative staining in Benign prostatic hyperplasia tissue.

    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) 使用論文

    This product has been referenced in:
    • Hoban AE  et al. Regulation of prefrontal cortex myelination by the microbiota. Transl Psychiatry 6:e774 (2016). WB ; Mouse . Read more (PubMed: 27045844) »
    • Michailidou I  et al. Complement C3 on microglial clusters in multiple sclerosis occur in chronic but not acute disease: Implication for disease pathogenesis. Glia N/A:N/A (2016). IHC-P ; Human . Read more (PubMed: 27778395) »

    See all 6 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver and brain)
    Gel Running Conditions
    Reduced Denaturing (4-20)
    Loading amount
    10 µg
    Specification
    liver and brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Isabelle Cote

    Verified customer

    投稿 Sep 28 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Primary oligodendrocytes differentiated for 5 days)
    Permeabilization
    No
    Specification
    Primary oligodendrocytes differentiated for 5 days
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 05 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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