Anti-Myelin oligodendrocyte glycoprotein 抗体 (ab32760)

製品の概要

  • 製品名Anti-Myelin oligodendrocyte glycoprotein antibody
    Myelin oligodendrocyte glycoprotein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myelin oligodendrocyte glycoprotein
  • アプリケーション適用あり: WB, IHC-FoFr, IPmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human, Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat Myelin oligodendrocyte glycoprotein.

    (Peptide available as ab32759.)

  • ポジティブ・コントロール
    • Mouse Brain Whole Tissue Lysate and Rat Brain Whole Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Rat Myelin oligodendrocyte glycoprotein peptide (ab32759).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22269040
IP Use a concentration of 5 µg/ml.

ターゲット情報

Anti-Myelin oligodendrocyte glycoprotein antibody 画像

  • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) at 1 µg/ml

    Lane 1 : Mouse Brain Whole Tissue Lysate
    Lane 2 : Rat Brain Whole Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Ab32760 used on Mouse Cortex and Hippocampus in free floating sections using direct fluorescence. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • Myelin oligodendrocyte glycoprotein was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Myelin oligodendrocyte glycoprotein and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32760.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 27kDa: Myelin oligodendrocyte glycoprotein

Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) 使用論文

This product has been referenced in:
  • Moczulska KE  et al. Deep and precise quantification of the mouse synaptosomal proteome reveals substantial remodeling during postnatal maturation. J Proteome Res 13:4310-24 (2014). WB ; Mouse . Read more (PubMed: 25157418) »
  • Ming X  et al. Transgenic overexpression of Sox17 promotes oligodendrocyte development and attenuates demyelination. J Neurosci 33:12528-42 (2013). Read more (PubMed: 23884956) »

See all 3 Publications for this product

Product Wall

Thank you so much for your call today and letting us know about the trouble with the vial of ab32760.

As we discussed, I'm sending a free of charge vial of the antibody on the order ***, which should arrive tomorrow.

Please keepme u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

投稿 Apr 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"