Immunocytochemistry/ Immunofluorescence - Anti-Myelin oligodendrocyte glycoprotein antibody (ab115597)This image is courtesy of an anonymous Abreview
ab115597 staining Myelin oligodendrocyte glycoprotein in rat oligodendrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/150) for 10 hour at 4°C. An Alexa Fluor® 594-conjugated donkey anti-goat IgG polyclonal (1/300) was used as the secondary antibody.
ab115597, at 5µg/ml, staining Myelin oligodendrocyte glycoprotein in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody (ab115597)
Anti-Myelin oligodendrocyte glycoprotein antibody (ab115597) at 0.03 µg/ml + Human Brain lysate at 30 µg developed using the ECL technique