Anti-Myelin Basic Protein 抗体 - Oligodendrocyte Marker (ab2404)

製品の概要

  • 製品名Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker
    Myelin Basic Protein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Myelin Basic Protein - Oligodendrocyte Marker
  • 特異性This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves.
  • アプリケーション適用あり: IHC-FoFr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Human myelin basic protein isolated from normal human brain.

  • ポジティブ・コントロール
    • Brain and spinal cord.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.
IHC-P 1/20 - 1/40. Antigen retrieval is not essential but may optimise staining.

(ABC method). We suggest an incubation period of 30 minutes at room temperature.

ターゲット情報

  • 機能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 組織特異性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 関連疾患Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 配列類似性Belongs to the myelin basic protein family.
  • 発生段階Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻訳後修飾Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 細胞内局在Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker 画像

  • ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30µm thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of 30 images taken with a 20x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
  • ab2404 staining Myelin Basic Protein in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry. Staining was detected using DAB.
  • ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

プロトコール

Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404) 使用論文

This product has been referenced in:
  • Bajaj A  et al. Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses. J Histochem Cytochem 61:19-30 (2013). Read more (PubMed: 23092790) »
  • Viertlboeck BC  et al. Chicken immunoregulatory Ig-like receptor families: an overview and expression details on ggTREM-A1. Dev Comp Immunol 41:403-12 (2013). Read more (PubMed: 23648646) »

See all 7 Publications for this product

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For ab2404, we list human and rat as reactive species as we guarantee that this antibody will react with those tissues, however we have not tested this antibody against any other tissue. Therefore there is a st...

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Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cells (primary))
Loading amount 30 µg
Specification Glioblastoma cells (primary)
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Dec 12 2011

I'm sorry to hear you are experiencing problems with ab2404. I think the problem might be due to the following: -the antibody may not recognise mouse MBP. The homology of the immunogen with mouse protein was found to only be 71%. It would be us...

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Thank you for your enquiry. Yes, Abcam's ab2404 Myelin Basic Protein antiserum can be applied using immunohistochemistry. We suggest antigen retrieval followed by an incubation period of 30 minutes at room temperature using a dilution range of 1/50...

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