Anti-Myelin Basic Protein 抗体 (ab123499)

製品の概要

  • 製品名Anti-Myelin Basic Protein antibody
    Myelin Basic Protein 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Myelin Basic Protein
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Mixture of 3 Human Myelin Basic Protein synthetic peptides, representing the different forms of the protein (alternate transcription from a single gene).

  • ポジティブ・コントロール
    • ICC/IF: Rat mixed neuron/glial cultures WB: Rat spinal cord whole tissue homogenate, or peripheral nerve homogenate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Predicted molecular weight: 33 kDa. A prominent band is often shown at about 20 kDa. This is a convergence of 3 different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa.
ICC/IF 1/1000.

ターゲット情報

  • 機能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 組織特異性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 関連疾患Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 配列類似性Belongs to the myelin basic protein family.
  • 発生段階Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻訳後修飾Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 細胞内局在Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody 画像

  • ab123499, at a 1/1000 dilution, staining Myelin Basic Protein in an oligodendrocyte from Rat mixed neuron/glial culture by Immunofluorescence.
  • Anti-Myelin Basic Protein antibody (ab123499) at 1/10000 dilution + whole tissue homogenate of Rat spinal cord

    Predicted band size : 33 kDa

Anti-Myelin Basic Protein antibody (ab123499) 使用論文

ab123499 has not yet been referenced specifically in any publications.

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