Anti-Myelin Basic Protein 抗体 [12] (ab92406)

製品の概要

  • 製品名
    Anti-Myelin Basic Protein antibody [12]
    Myelin Basic Protein 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [12] to Myelin Basic Protein
  • 由来種
    Rat
  • 特異性
    Myelin basic protein
  • アプリケーション
    適用あり: ELISA, IHC-Fr, RIAmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide (Cow)

  • エピトープ
    ab92406 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IHC-Fr
RIA
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • 組織特異性
      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • 関連疾患
      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • 配列類似性
      Belongs to the myelin basic protein family.
    • 発生段階
      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • 翻訳後修飾
      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • 細胞内局在
      Myelin membrane. Cytoplasmic side of myelin.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_CAVPO antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin A1 Protein, basic antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    プロトコール

    参考文献

    ab92406 has not yet been referenced specifically in any publications.

    レビューと Q&A

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Xenopus laevis Tissue sections (optic nerve)
    Specification
    optic nerve
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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    投稿 May 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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