Anti-Myelin Basic Protein 抗体 [12] (ab7349)


  • 製品名
    Anti-Myelin Basic Protein antibody [12]
    Myelin Basic Protein 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [12] to Myelin Basic Protein
  • 特異性
    The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Mapped by Geyson method to DENPW method.
  • アプリケーション
    適用あり: IHC-FoFr, IHC-P, WB, ELISA, RIA, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Apteronotus leptorhynchus
  • 免疫原

    Full length protein (Cow).

  • エピトープ
    Amino acids 82-87 (DENPVV).



Our Abpromise guarantee covers the use of ab7349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 18678887
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23584610


  • 機能
    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 組織特異性
    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 関連疾患
    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 配列類似性
    Belongs to the myelin basic protein family.
  • 発生段階
    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻訳後修飾
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 細胞内局在
    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all


  • Anti-Myelin Basic Protein antibody [12] (ab7349) + Mouse brain tissue lysate

    Observed band size : 19,26 kDa (why is the actual band size different from the predicted?)
  • ab7349 staining Myelin Basic Protein in human trigeminal ganglia tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/1000 in 10% NDS) for 16 hours at 22°C. ab150165, a goat anti-rat IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab7349 staining Myelin Basic Protein in murine cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was not fixed. Samples were blocked with 2.5% serum for 1 hour followed by incubation with the primary antibody at a 1/200 dilution for 18 hours. A Biotin-conjugated rabbit anti-rat polyclonal was used as the secondary antibody at a 1/500 dilution.
  • ab7349 at 1/100 staining mouse spinal cord tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum prior to incubation with the antibody for 18 hours. A biotinylated horse anti-rat antibody was used as the secondary.

    See Abreview


This product has been referenced in:
  • Carusillo Theriault B  et al. Cerebral microbleeds in a neonatal rat model. PLoS One 12:e0171163 (2017). IHC-Fr ; Rat . Read more (PubMed: 28158198) »
  • Al-Ghobashy MA  et al. Development and Pre-Clinical Evaluation of Recombinant Human Myelin Basic Protein Nano Therapeutic Vaccine in Experimental Autoimmune Encephalomyelitis Mice Animal Model. Sci Rep 7:46468 (2017). Read more (PubMed: 28425447) »

See all 65 Publications for this product

レビューと Q&A

Thank you for your enquiry. I do not have the accession number for the immunogen; however, the amino acid sequence to which the antibody binds is DENPVV. I have updated the datasheet for future reference. I hope this information helps, please do...

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IHC - Wholemount
Mouse Tissue (Brain, striatum)
Brain, striatum

Abcam user community

Verified customer

投稿 May 23 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Trigeminal Ganglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Trigeminal Ganglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

April Rempel

Verified customer

投稿 May 13 2015

Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: Rm°C
Human Tissue sections (Human Myelin/Oligodendrocytes in Mouse Shiverer Br)
Human Myelin/Oligodendrocytes in Mouse Shiverer Br
Yes - Triton

Dr. Fraser Sim

Verified customer

投稿 Sep 16 2014

Immunohistochemistry (PFA perfusion fixed frozen sections)
Zebra finch Tissue sections (Brain)
Antigen retrieval step
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Mr. Adam Piraino

Verified customer

投稿 Mar 28 2013

Thank you for your reply.

The following antibodies are suitable for your primary antibody ab7349 which is a rat IgG2a:


ab99668 (or use the following: ...

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Before I can recommend a secondary antibody to you, I would need to know the following:

What application are you using, IHC-P or IHC-Fr?

Shall the antibody be conjugated with an enzyme or with a Fluorochrom?

Will you use the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 23 2013

Thank you for your enquiry.

I can confirm that the anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker ab7349 is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue c...

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Unfortunately there is no estimate for the purity level.
The use of unpurified antibody should not interefere with the experiements.

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