MyD88 overexpression 293T lysate (whole cell) (ab94067)

製品の概要

  • 製品名
    MyD88 overexpression 293T lysate (whole cell)
    MyD88 ライゼート 製品一覧
  • 製品の詳細
    MyD88 overexpression lysate
  • 特記事項
    ab94067 is a 293T cell transfected lysate in which Human MyD88 has been transiently over-expressed using a pCMV-MyD88 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Tissue specificity: Ubiquitous. Disease: Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. Similarity: Contains 1 death domain. Contains 1 TIR domain. Domain: The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

アプリケーション

Our Abpromise guarantee covers the use of ab94067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94067 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-MyD88 antibody (ab89576) at 1/500 dilution

    Lane 1 : MyD88 overexpression 293T lysate (whole cell) (ab94067) at 25 µg
    Lane 2 : 293T non-transfected lysate

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94067 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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