製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MyD88
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Human MyD88 (internal sequence). ab2068 antibody was raised against a 16 amino acid peptide near the center of human MD88
    (Peptide available as ab7882)

  • ポジティブ・コントロール
    • wB: Jurkat whole cell lysate IHC-P: human heart tissue
  • 特記事項
    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. My D88 gene is expressed in many tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa.Can be blocked with Human MyD88 peptide (ab7882).

Use a modified RIPA lysis buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Remove cell debris by centrifugation. Boil the lysate for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol). Block the membrane with 5% non-fat dry milk in TBS overnight at 4°C or 2 hours at room temperature.

Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 配列類似性
    Contains 1 death domain.
    Contains 1 TIR domain.
  • ドメイン
    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

画像

  • Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab2068 staining MyD88 in Human sigma / diverticulitis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. An undiluted Biotin-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody

    See Abreview

  • ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
  • Immunofluorescence of MYD88 in Human Testis cells using ab2068 at 20 ug/ml.

参考文献

This product has been referenced in:
  • Guo J  et al. LPS/TLR4-mediated stromal cells acquire an invasive phenotype and are implicated in the pathogenesis of adenomyosis. Sci Rep 6:21416 (2016). IHC-P ; Human . Read more (PubMed: 26898650) »
  • Wen Y  et al. Acetylbritannilactone Modulates MicroRNA-155-Mediated Inflammatory Response in Ischemic Cerebral Tissues. Mol Med 21:197-209 (2015). Read more (PubMed: 25811992) »

See all 13 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Mouse Cell lysate - whole cell (Mouse bone marrow-derived macrophages)
Specification
Mouse bone marrow-derived macrophages
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Sep 02 2013

Thank you for contacting us. Wehave not yet tested this in rat tissue but the epitope matches the rat sequence 100% so it is likely to work. Please let me know if you have any further questions.

A refund has been issued.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (sigma / diverticulitis)
Specification
sigma / diverticulitis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH6
Permeabilization
No
Username

Dr. Ilka Werner-Martini

Verified customer

投稿 Oct 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Macrophage)
Loading amount
10 µg
Specification
Macrophage
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 18 2008

All the information that we have regarding these two antibodies is located on the online datasheets. Both have been tested for application in Western blotting and shown to cross-react with human and mouse, but I really can't say which one works "best."

Thank you very much for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets. Ab2064 corresponds to amino acids 279-296 of Human MyD88 (epitope is CTKSWFWTRLAKALSLP). Ab2068 corresponds to am...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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