Anti-MyD88 抗体 [4D6] (ab107585)


  • 製品名
    Anti-MyD88 antibody [4D6]
    MyD88 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4D6] to MyD88
  • 特異性
    Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • ポジティブ・コントロール
    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells.



Our Abpromise guarantee covers the use of ab107585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


  • 機能
    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 組織特異性
  • 関連疾患
    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 配列類似性
    Contains 1 death domain.
    Contains 1 TIR domain.
  • ドメイン
    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 細胞内局在
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all


  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
    ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
  • Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
  • All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

    Lane 1 : Human ovary cell lysate
    Lane 2 : Human prostate cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Jurkat cell lysate with Immunizing peptide

    Goat anti-mouse Ig HRP

    Predicted band size : 33 kDa


ab107585 has not yet been referenced specifically in any publications.

レビューと Q&A

Ablaufdatum: 01 März. 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab107585 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Guts...

Read More