Anti-Mycobacterium tuberculosis Ag85 抗体 (ab36731)


  • 製品名Anti-Mycobacterium tuberculosis Ag85 antibody
  • 製品の詳細
    Mouse monoclonal to Mycobacterium tuberculosis Ag85
  • 特異性This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
  • 免疫原

    Bacterial press extract from Mycobacterium tuberculosis H37Rv

  • エピトープEpitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
  • 特記事項

    WHO no. IT-49


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野


Our Abpromise guarantee covers the use of ab36731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
WB Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.


  • 関連性Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
  • 別名
    • Antigen 85 complex A antibody
    • Antigen 85 complex B antibody
    • Antigen 85 complex C antibody
    • fbpA antibody
    • fbpB antibody
    • fbpC antibody
    • Secreted antigen 85 A antibody
    • Secreted antigen 85 B antibody
    • Secreted antigen 85 C antibody
    see all

Anti-Mycobacterium tuberculosis Ag85 antibody (ab36731) 使用論文

This product has been referenced in:
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »
  • Rengarajan J  et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . Read more (PubMed: 18172199) »

See all 4 Publications for this product

Product Wall

HYT 27, does react in WB with both Ag85A, B and C, see the paper by Armitige LY and colleagues, whom used HYT 27 in WB (on page 774, fbpA antibody) and showed very good reaction with A.

Armitage et al., INFECTION AND IMM...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding the specificity and cross reactivity of ab36731.


HYT 27 is specific for Mycobacterium mycolyltransferase. ...

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Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (transformed E coli)
Loading amount 0.01 µg
Specification transformed E coli
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

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投稿 Aug 29 2007