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EQKLISEEDL (C-MYC) conjugated with KLH.
Our Abpromise guarantee covers the use of ab9106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19793804
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 16093346|
|ICC||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).|
ab9106 at 4µg/mg lysate used in human huh-7 whole cell lysate (1x106 cells). Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.
Ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate). In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.
Lane 1: Input untransfected cells
Lane 2: Immunoprecipitated untransfected cells
Lane 3: Input co-transfected cells
Lane 4: Immunoprecipitated co-transfected cells
This image is courtesy of an Abreview submitted on 23 March 2006.