製品の概要
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製品名Anti-Myc tag antibody
Myc tag 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Myc tag
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由来種Goat
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アプリケーション適用あり: WBmore details
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免疫原
Synthetic peptide: C-EQKLISEEDL conjugated to KLH, corresponding to Myc tag.
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ポジティブ･コントロール
- This antibody gave a positive signal against an E. coli lysate that expresses a 41kDa recombinant protein comprised of 11 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab107247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. |
ターゲット情報
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関連性Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
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細胞内局在Nuclear
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別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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Western blotAnti-Myc tag antibody (ab107247) at 1 µg/ml +E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 41 kDa (why is the actual band size different from the predicted?)
This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 11 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
プロトコール
参考文献
ab107247 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"