Anti-Myc tag 抗体 [9E10] (Phycoerythrin) (ab72468)

製品の概要

  • 製品名
    Anti-Myc tag antibody [9E10] (Phycoerythrin)
    Myc tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9E10] to Myc tag (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 免疫原

    Synthetic peptide within Human c-Myc aa 408-432 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    AEEQKLISEEDLLRKRREQLKHKLE

  • 特記事項

    Conjugate = SureLight® R-Phycoerythrin.

    We recommend that the investigator determine the appropriate working concentration for their specific application.

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

Visible absorption maxima 565>540>498

Emission maximum 578





ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 細胞内局在
    Nuclear
  • 別名
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

画像

  • Fluorescence excitation and emission spectra of R-phycoerythrin in 100 mM sodium phosphate (pH 7.2) + 1mM EDTA and 1 mM sodium azide. Emission scan was taken with excitation at 498 nm. Excitation scan was taken with emission t 575 nm. Scans were normalized to equalize peak heights.

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参考文献

ab72468 has not yet been referenced specifically in any publications.

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