Mouse monoclonal [9E10] to Myc tag (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Visible absorption maxima 565>540>498
Emission maximum 578
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Flow Cytometry - Anti-Myc tag antibody [9E10] (Phycoerythrin) (ab72468)
Fluorescence excitation and emission spectra of R-phycoerythrin in 100 mM sodium phosphate (pH 7.2) + 1mM EDTA and 1 mM sodium azide. Emission scan was taken with excitation at 498 nm. Excitation scan was taken with emission t 575 nm. Scans were normalized to equalize peak heights.