Anti-MYBPC1 抗体 (ab110363)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MYBPC1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human MYBPC1.

  • ポジティブ・コントロール
    • 293 cell extract.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab110363 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.

ターゲット情報

  • 機能
    Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. MyBP family.
    Contains 3 fibronectin type-III domains.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C protein, skeletal muscle slow isoform antibody
    • C-protein antibody
    • MYBPC1 antibody
    • MYBPCC antibody
    • MYBPCS antibody
    • Myosin binding protein C, slow type antibody
    • Myosin-binding protein C antibody
    • MYPC1_HUMAN antibody
    • skeletal muscle C protein antibody
    • skeletal muscle slow isoform antibody
    • Slow MyBP C antibody
    • Slow MyBP-C antibody
    • slow-type antibody
    see all

Anti-MYBPC1 antibody 画像

  • All lanes : Anti-MYBPC1 antibody (ab110363) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 128 kDa

プロトコール

Anti-MYBPC1 antibody (ab110363) 使用論文

ab110363 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 22 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4%-12%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 31 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
WB Blocking Solution (commercial) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Brian Lin

Verified customer

投稿 Jul 23 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
WB blocking buffer as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Brian Lin

Verified customer

投稿 Jul 14 2014

Thank you for your inquiry. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. I can state that the immunogen peptide amino acid range for this product is xxxxxx of Human ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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