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Immunogen corresponds to a region within amino acids 450-500 of Human MYBBP1A (NP_055335.1).
Our Abpromise guarantee covers the use of ab99361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at 2-5 µg/mg of lysate.|
ICC/IF image of ab99361 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99361 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) HepG2, HEk293, and MCF-7 cells at 1ug/ml.
Western Blot: rabbit anti-MYBBP1A antibody at 1 µg/ml
developed using the ECL technique
Exposure time : 1 second
Predicted band size : 149 kDa
ab99361 has not yet been referenced specifically in any publications.