製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MX1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Monkey
    交差が予測される動物種: Dog
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 246 of Human MX1.

  • ポジティブ・コントロール
    • WB: H1299, HeLa, and Raji whole cell lysate; 293T, A431 and HepG2 cell lysates IHC-P: MDA-MB-468 xenograft

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by antigen affinity chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus.
  • 配列類似性
    Belongs to the dynamin family.
    Contains 1 GED domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Human interferon regulated resistance GTP binding protein MXA antibody
    • IFI 78 antibody
    • IFI 78K antibody
    • IFI-78K antibody
    • IFI78 antibody
    • IFI78K antibody
    • Interferon induced GTP binding protein Mx1 antibody
    • Interferon induced protein p78 antibody
    • Interferon inducible protein p78 antibody
    • Interferon regulated resistance GTP binding protein antibody
    • Interferon regulated resistance GTP binding protein MxA antibody
    • Interferon-induced GTP-binding protein Mx1 antibody
    • Interferon-induced protein p78 antibody
    • Interferon-regulated resistance GTP-binding protein MxA antibody
    • MX 1 antibody
    • MX antibody
    • MX dynamin-like GTPase 1 antibody
    • MX1 antibody
    • MX1_HUMAN antibody
    • MxA antibody
    • Myxoma resistance protein 1 antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible antibody
    • Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 antibody
    • Myxovirus (influenza) resistance 1 homolog of murine antibody
    • Myxovirus resistance 1, mouse, homolog of antibody
    • Myxovirus resistance protein 1 antibody
    • N-terminally processed antibody
    see all

Anti-MX1 antibody 画像

  • Lane 1 : Anti-MX1 antibody (ab95926) at 1/1000 dilution
    Lane 2 : Anti-MX1 antibody (ab95926)

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : Human MX-1 transfected 293T cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size : 76 kDa
  • Immunohistochemical analysis of paraffin embedded MDA-MB-468 xenograft, using ab95926 at a 1/500 dilution.
  • All lanes : Anti-MX1 antibody (ab95926) at 1/5000 dilution

    Lane 1 : H1299 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 76 kDa

Anti-MX1 antibody (ab95926) 使用論文

This product has been referenced in:
  • Lee SJ  et al. Expression of Myxovirus Resistance A (MxA) Is Associated with Tumor-Infiltrating Lymphocytes in Human Epidermal Growth Factor Receptor 2 (HER2)-Positive Breast Cancers. Cancer Res Treat 49:313-321 (2017). IHC ; Human . Read more (PubMed: 27456948) »
  • Sullivan KD  et al. Trisomy 21 consistently activates the interferon response. Elife 5:N/A (2016). Read more (PubMed: 27472900) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chimpanzee Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Fixative
Formaldehyde
Username

Dr. Edward Greenwood

Verified customer

投稿 Feb 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
African Green Monkey Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Lymph Node
Blocking step
No blocking step used for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 25 2016

Thank you for contacting us.

To our knowledge, these products have not been tested for cross reactivity with Mx2. As the homology between the two protein is only 55%, we would not assume any cross reactivity however as this is not tested w...

Read More

Thank you for contacting us.
The recommended Hela cell lysates are positive control for WB.
For more information about IHC-P positive control, please click the following link;
http://www.proteinatlas.org/ENSG00000157601/cancer
I hope ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (inflamed spleen (monkey))
Specification
inflamed spleen (monkey)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Blocking step
Phosphate buffer containing hydrogen peroxide, 15 mmol/L NaN3 and detergent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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