製品の概要

  • 製品名Anti-MVP antibody
    MVP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MVP
  • アプリケーション適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Cat
  • 免疫原

    Recombinant fragment: EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS, corresponding to amino acids 158-350 of Human MVP (AAH15623)

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, A549 and Raji cells

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 組織特異性Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 配列類似性Contains 9 MVP (vault) repeats.
  • ドメインMVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻訳後修飾Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 細胞内局在Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Anti-MVP antibody 画像

  • Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97311 at 1/500 dilution.

Anti-MVP antibody (ab97311) 使用論文

This product has been referenced in:
  • O'Dwyer D  et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . Read more (PubMed: 22125622) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal Muscle)
Loading amount 30 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jun 30 2012

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