Anti-MVP 抗体 (ab97311)
Key features and details
- Rabbit polyclonal to MVP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MVP antibody
MVP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MVP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Cat -
免疫原
Recombinant fragment corresponding to Human MVP aa 158-350.
Sequence:EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS
Database link: Q14764 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97311の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. -
組織特異性
Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells. -
配列類似性
Contains 9 MVP (vault) repeats. -
ドメイン
MVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4. -
翻訳後修飾
Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11. -
細胞内局在
Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 9961 Human
- Entrez Gene: 78388 Mouse
- Entrez Gene: 64681 Rat
- Omim: 605088 Human
- SwissProt: Q14764 Human
- SwissProt: Q9EQK5 Mouse
- SwissProt: Q62667 Rat
- Unigene: 632177 Human
see all -
別名
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
see all
画像
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All lanes : Anti-MVP antibody (ab97311) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : MVP Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 99 kDa -
Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 99 kDa
7.5% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded A549 xenograft using ab97311 at 1/500 dilution.
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All lanes : Anti-MVP antibody (ab97311) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : BCL-1 whole cell lysate
Lane 5 : Raw264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 99 kDa7.5% SDS-PAGE
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A431 cells stained for MVP/LRP (green) using ab97311 at 1/200 dilution in ICC/IF.
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Paraffin embedded mouse colon tissue stained for MVP/LRP using ab97311 at 1/500 dilution in immunohistochemical analysis.
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Paraffin embedded human breast carcinoma tissue stained for MVP/LRP using ab97311 at 1/500 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97311 は 2 報の論文で使用されています。
- Shi L et al. Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer 17:138 (2017). WB . PubMed: 28202053
- O'Dwyer D et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . PubMed: 22125622