製品の概要

  • 製品名
    Anti-MVP antibody [1032]
    MVP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1032] to MVP
  • 特異性
    This antibody reacts with a 104 to 110 kD protein known as Major Vault Protein (MVP).
  • アプリケーション
    適用あり: WB, IP, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

  • ポジティブ・コントロール
    • Breast.
  • 特記事項


    MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.
  • ポリ/モノ
    モノクローナル
  • クローン名
    1032
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 組織特異性
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 配列類似性
    Contains 9 MVP (vault) repeats.
  • ドメイン
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻訳後修飾
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 細胞内局在
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Anti-MVP antibody [1032] 画像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining MVP with ab2376.
  • ab2376 - immunohistochemistry

    Formalin fixed paraffin embedded human breast carcinoma stained with MVP, using ABC and DAB chromogen.

Anti-MVP antibody [1032] (ab2376) 使用論文

This product has been referenced in:
  • Durcin M  et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles 6:1305677 (2017). WB ; Mouse . Read more (PubMed: 28473884) »
  • Xu H  et al. Knock-down of ubiquitin-specific protease 22 by micro-RNA interference inhibits colorectal cancer growth. Int J Colorectal Dis : (2011). WB ; Human . Read more (PubMed: 21773699) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - other (Lung, endothelial cells)
Gel Running Conditions
Reduced Denaturing (7.5 %)
Loading amount
40 µg
Specification
Lung, endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Monica Giannotta

Verified customer

投稿 Jul 21 2015

I am very pleased to hear you would like to accept our offer and test ab2376 in Western blot on mouse. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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