製品の概要

  • 製品名
    Anti-MVP antibody [1032]
    MVP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1032] to MVP
  • 特異性
    The clone number has been updated from (0.N.389) to (1032) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: Flow Cyt, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    1032
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 104-110 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.
EMSA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 組織特異性
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 配列類似性
    Contains 9 MVP (vault) repeats.
  • ドメイン
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻訳後修飾
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 細胞内局在
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

参考文献

This product has been referenced in:
  • Fowler PA  et al. In utero exposure to cigarette smoke dysregulates human fetal ovarian developmental signalling. Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24847019) »
  • Bellingham M  et al. Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. Mol Cell Endocrinol 376:156-72 (2013). IHC . Read more (PubMed: 23791816) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - other (monocytes)
Specification
monocytes
Blocking step
Roti-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

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投稿 Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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