製品の概要

  • 製品名Anti-MVP antibody [0.N.389]
    MVP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [0.N.389] to MVP
  • アプリケーション適用あり: IHC-P, WB, Flow Cyt, IPmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • ポジティブ・コントロール
    • SKBR3 cells and breast carcinomas

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名0.N.389
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
Flow Cyt
IP
EMSA
  • 追加情報Flow Cyt: Use at an assay dependent dilution.
    GSA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT (staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
    IP: Use 2µg/mg protein lysate (Use Protein G).
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hrs at RT. Predicted molecular weight: 110 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
    • 組織特異性Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
    • 配列類似性Contains 9 MVP (vault) repeats.
    • ドメインMVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.
    • 翻訳後修飾Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.
    • 細胞内局在Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt
    • 参照データベース
    • 別名
      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    Anti-MVP antibody [0.N.389] (ab14563) 使用論文

    ab14563 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab14563.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"