製品の概要

  • 製品名Anti-Muscleblind-like 1 antibody
    Muscleblind-like 1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Muscleblind-like 1
  • 特異性ab80599 is expected to recognize the reported isoforms a - f (NP_066368.2, NP_997175.1, NP_997176.1, NP_997177.1, NP_997178.1 and NP_997179.1)
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-SAEHLTSHKYVTQ

    , corresponding to C terminal amino acids 375 - 387 of Human Muscleblind-like 1

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab80599 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報Peptide ELISA: antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 41 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
    • 組織特異性Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 関連疾患Plays a role in the pathogenesis of dystrophia myotonica type 1 (DM1) [MIM:160900]. A muscular disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness and cardiac arrhythmias. Note=In muscle cells from DM1 patients, MBNL1 is sequestered by DMPK RNAs containing CUG triplet repeat expansions. MBNL1 binding is proportional to repeat length consistent with the direct correlation between the length of repeat expansion and disease severity.
    • 配列類似性Belongs to the muscleblind family.
      Contains 4 C3H1-type zinc fingers.
    • 細胞内局在Nucleus. Cytoplasm. Cytoplasmic granule. Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress. Localized in the cytoplasm of multinucleated myotubes. Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EXP antibody
      • EXP35 antibody
      • EXP40 antibody
      • EXP42 antibody
      • KIAA0428 antibody
      • MBNL antibody
      • MBNL protein antibody
      • MBNL1 antibody
      • MBNL1_HUMAN antibody
      • Muscleblind 41kD isoform antibody
      • Muscleblind like antibody
      • Muscleblind like protein 1 antibody
      • Muscleblind like splicing regulator 1 antibody
      • Muscleblind-like protein 1 antibody
      • Triplet expansion RNA binding protein antibody
      • Triplet-expansion RNA-binding protein antibody
      see all

    Anti-Muscleblind-like 1 antibody 画像

    • Anti-Muscleblind-like 1 antibody (ab80599) at 1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 41 kDa
      Observed band size : 41 kDa

    Anti-Muscleblind-like 1 antibody (ab80599) 使用論文

    ab80599 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab80599.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"