製品の概要

  • 製品名Anti-Muscleblind-like 1 antibody
    Muscleblind-like 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Muscleblind-like 1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    A region within synthetic peptide: QVFPSLQQPQ PTSPILDAST LLGATSCPAA AGKMIPIISA EHLTSHKYVT, corresponding to C terminal amino acids 289-338 of Human Muscleblind-like 1

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
  • 組織特異性Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 関連疾患Plays a role in the pathogenesis of dystrophia myotonica type 1 (DM1) [MIM:160900]. A muscular disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness and cardiac arrhythmias. Note=In muscle cells from DM1 patients, MBNL1 is sequestered by DMPK RNAs containing CUG triplet repeat expansions. MBNL1 binding is proportional to repeat length consistent with the direct correlation between the length of repeat expansion and disease severity.
  • 配列類似性Belongs to the muscleblind family.
    Contains 4 C3H1-type zinc fingers.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasmic granule. Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress. Localized in the cytoplasm of multinucleated myotubes. Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EXP antibody
    • EXP35 antibody
    • EXP40 antibody
    • EXP42 antibody
    • KIAA0428 antibody
    • MBNL antibody
    • MBNL protein antibody
    • MBNL1 antibody
    • MBNL1_HUMAN antibody
    • Muscleblind 41kD isoform antibody
    • Muscleblind like antibody
    • Muscleblind like protein 1 antibody
    • Muscleblind like splicing regulator 1 antibody
    • Muscleblind-like protein 1 antibody
    • Triplet expansion RNA binding protein antibody
    • Triplet-expansion RNA-binding protein antibody
    see all

Anti-Muscleblind-like 1 antibody 画像

  • Lane 1 : Marker
    Lane 2 : Anti-Muscleblind-like 1 antibody (ab50635) at 2.5 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 37 kDa

Anti-Muscleblind-like 1 antibody (ab50635) 使用論文

ab50635 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"