製品の概要

  • 製品名Anti-muscle Actin antibody [EP184E]
    muscle Actin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP184E] to muscle Actin
  • 特異性This Actin antibody recognizes all muscle actin.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Actin.

  • ポジティブ・コントロール
    • A431 cell lysate; HeLa cells; human cardiac muscle
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-muscle Actin antibody (HRP) [EP184E] (ab197716)

    Anti-muscle Actin antibody (Alexa Fluor® 488) [EP184E] (ab197850)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • 特記事項(精製)This antibody has not been purified. It is provided in cell supernatant and storage buffer.
  • ポリ/モノモノクローナル
  • クローン名EP184E
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab46805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr
IHC-P
ICC/IF
Flow Cyt
IP
WB
  • 追加情報Flow Cyt: 1/20.
    ICC/IF: 1/250 - 1/500.
    IHC-P: 1/100 - 1/200.
    IHC-FoFr: 1/500 (PMID 18835925).
    IP: 1/20.
    WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • 関連疾患Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • 配列類似性Belongs to the actin family.
    • 翻訳後修飾Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • ACTA2 antibody
      • ACTC antibody
      • ACTC1 antibody
      • Actin antibody
      • ACTS_HUMAN antibody
      • ACTSA antibody
      • Alpha 2 actin antibody
      • alpha skeletal muscle antibody
      • Alpha-actin-1 antibody
      • Cardiac muscle alpha actin 1 antibody
      • Skeletal muscle alpha actin 1 antibody
      see all

    Anti-muscle Actin antibody [EP184E] 画像

    • Anti-muscle Actin antibody [EP184E] (ab46805) at 1/1000 dilution + A431 cell lysate at 10ug/lane

      Secondary
      Goat anti-rabbit, HRP labelled. at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
    • Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin ab46805 at a 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.

    • Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with ab46805.

    Anti-muscle Actin antibody [EP184E] (ab46805) 使用論文

    This product has been referenced in:
    • Li Q  et al. Phosphoinositide 3-kinase p110d mediates estrogen- and FSH-stimulated ovarian follicle growth. Mol Endocrinol 27:1468-82 (2013). WB ; Mouse . Read more (PubMed: 23820902) »
    • Zachut M  et al. Periparturient dairy cows do not exhibit hepatic insulin resistance, yet adipose-specific insulin resistance occurs in cows prone to high weight loss. J Dairy Sci 96:5656-69 (2013). Cow . Read more (PubMed: 23871373) »

    See all 7 Publications for this product

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