Anti-Muscarinic Acetylcholine Receptor M5 抗体 (ab41171)

製品の概要

  • 製品名Anti-Muscarinic Acetylcholine Receptor M5 antibody
    Muscarinic Acetylcholine Receptor M5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5 - Carboxyterminal end
  • 特異性Cross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor
  • アプリケーション適用あり: WB, ELISA, IHC (PFA fixed), IHC-FoFrmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Synthetic peptide analogue of the C terminal of rat muscarinic M5 receptor covalently attached onto a carrier protein.

  • ポジティブ・コントロール
    • Rat brain tissue, whole brain homogenate and cultured cells.

製品の特性

  • 製品の状態Lyophilised:Reconstitute the lyophilized pooled antisera with 0.1 ml of 10 mg/ml BSA and 0.01% Sodium Azide in PBS for the equivalent of whole antisera, or with additional buffer for more dilute antisera.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab41171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/400. Predicted molecular weight: 60 kDa. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.
ELISA Use at an assay dependent concentration.
IHC (PFA fixed) 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23773170

ターゲット情報

  • 関連性The muscarinic acetylcholine receptor M5 is a member of the GPCR family (subfamily, acetylcholine) which mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. The rimary transducing effect is PI turnover. The receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.
  • 細胞内局在Membrane; multi-pass membrane protein.
  • 参照データベース
  • 別名
    • Acetylcholine receptor muscarinic 5 antibody
    • AChR antibody
    • AChR M5 antibody
    • CHKM5MR antibody
    • Cholinergic receptor muscarinic 5 antibody
    • Chrm 5 antibody
    • CHRM5 antibody
    • HM 5 antibody
    • HM5 antibody
    • m5 antibody
    • M5R antibody
    • MGC41838 antibody
    see all

Anti-Muscarinic Acetylcholine Receptor M5 antibody (ab41171) 使用論文

This product has been referenced in:
  • Wasserman DI  et al. Cholinergic control of morphine-induced locomotion in rostromedial tegmental nucleus versus ventral tegmental area sites. Eur J Neurosci 38:2774-85 (2013). IHC-FoFr ; Mouse . Read more (PubMed: 23773170) »

See 1 Publication for this product

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I've looked through our records andto our knowledge, the antibody has only been tested inWestern blot, ELISA, and IHC on standard PFA-fixed tissue. I will get in touch with the lab and request i...

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