Anti-Muscarinic Acetylcholine Receptor M3 抗体 (ab87199)

製品の概要

  • 製品名
    Anti-Muscarinic Acetylcholine Receptor M3 antibody
    Muscarinic Acetylcholine Receptor M3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Sheep, Rabbit, Horse, Cow, Pig, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Rat Muscarinic Acetylcholine Receptor M3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Rat tissue lysates: Brain; Forebrain; Substantia Nigra; Heart.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
  • 細胞内局在
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AChR antibody
    • ACM3_HUMAN antibody
    • cholinergic receptor muscarinic 3 antibody
    • CHRM 3 antibody
    • CHRM3 antibody
    • EGBRS antibody
    • HM 3 antibody
    • HM3 antibody
    • m3 muscarinic acetylcholine receptor antibody
    • M3 muscarinic receptor antibody
    • muscarinic 3 antibody
    • Muscarinic acetylcholine receptor M3 antibody
    • muscarinic cholinergic m3 receptor antibody
    • muscarinic m3 receptor antibody
    see all

画像

  • All lanes : Anti-Muscarinic Acetylcholine Receptor M3 antibody (ab87199) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain Rat Tissue Lysate
    Lane 3 : Rat Substantia Nigra Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of Muscarinic Acetylcholine Receptor M3 staining in Rat normal brain (Sagittal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87199, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Wilson C  et al. Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium. FASEB J 30:2000-13 (2016). ICC . Read more (PubMed: 26873937) »
  • Eckhard A  et al. Regulation of the perilymphatic-endolymphatic water shunt in the cochlea by membrane translocation of aquaporin-5. Pflugers Arch 467:2571-88 (2015). IF . Read more (PubMed: 26208470) »

See all 2 Publications for this product

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