Anti-muscarinic Acetylcholine Receptor 1 抗体 (ab75178)


  • 製品名Anti-muscarinic Acetylcholine Receptor 1 antibody
    Muscarinic Acetylcholine Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to muscarinic Acetylcholine Receptor 1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from the internal region of human Muscarinic Acetylcholine Receptor 1

  • ポジティブ・コントロール
    • Extracts from HepG2 cells



Our Abpromise guarantee covers the use of ab75178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/5000.


  • 関連性The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine.
  • 細胞内局在Cell Membrane; multi-pass membrane protein
  • 参照データベース
  • 別名
    • AChR antibody
    • Cholinergic receptor muscarinic 1 antibody
    • CHRM1 antibody
    • HM1 antibody
    • M1 antibody
    • Muscarinic acetylcholine receptor M1 antibody
    see all

Anti-muscarinic Acetylcholine Receptor 1 antibody 画像

  • All lanes : Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178) 使用論文

This product has been referenced in:
  • Falsafi SK  et al. Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems. PLoS One 7:e32082 (2012). WB ; Mouse . Read more (PubMed: 22384146) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Sample Mouse Cell (Brain)
Specification Brain
Permeabilization Yes - 0.5% Triton X 100 in PBS
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, membrane fraction)
Loading amount 50 µg
Specification brain tissue lysate, membrane fraction
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Abcam user community

Verified customer

投稿 Apr 12 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

Read More

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application o...

Read More