Anti-Musashi 1 / Msi1 抗体 [EP1302] (ab52865)

製品の概要

  • 製品名Anti-Musashi 1 / Msi1 antibody [EP1302]
    Musashi 1 / Msi1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
  • 特異性Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
  • アプリケーション適用あり: IHC-P, ICC/IF, IHC-Fr, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Chicken, Human, Quail
  • 免疫原

    corresponding to Human Musashi 1/ Msi1 (N terminal).
    (Peptide available as ab178003)

  • ポジティブ・コントロール
    • PC12 cells, SH-SY-5Y cell lysate and human brain tissue.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IHC-Fr 1/100.
WB 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Musashi 1 / Msi1 peptide (ab178003).
IP Use at an assay dependent concentration.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 組織特異性Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • 配列類似性Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • ドメインThe first RNA recognition motif binds more strongly to RNA compared to the second one.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Msi 1 antibody
    • Msi1 antibody
    • MSI1H_HUMAN antibody
    • Musashi homolog 1 antibody
    • Musashi-1 antibody
    • Musashi1 antibody
    • RNA binding protein Musashi homolog 1 antibody
    • RNA-binding protein Musashi homolog 1 antibody
    see all

Anti-Musashi 1 / Msi1 antibody [EP1302] 画像

  • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

  • Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution + SH-SY-5Y cell lysate at 10 µg/ml

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
  • Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized. The cells were incubated with ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.

    See Abreview

  • Musashi 1 / Msi1 was immunoprecipitated using 0.5mg SHSY5Y whole cell extract, 10µg of Rabbit monoclonal to Musashi 1 / Msi1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, SHSY5Y whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52865.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 39kDa: Musashi 1 / Msi1; non specific - 29kDa: We are unsure as to the identity of this extra band.

Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) 使用論文

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
  • Dorfman T  et al. Enhanced intestinal epithelial cell proliferation in diabetic rats correlates with ß-catenin accumulation. J Endocrinol 226:135-43 (2015). Read more (PubMed: 26297291) »

See all 11 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated
Sample Human Tissue sections (Teratoma)
Specification Teratoma
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 19 2015

Application Immunohistochemistry (Frozen sections)
Sample Xenopus laevis Tissue sections (CNS)
Specification CNS
Fixative Formaldehyde
Permeabilization Yes - 1% Triton X-100
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Gufa Lin

Verified customer

投稿 Apr 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Leica Bond Epitope Retrieval 2
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Apr 02 2013

Thank you very much for your interest our products.

To our knowledge, neither ab21628 nor ab52865 have been tested in Xenopus. The homology of the immunogen of ab21628 with Xenopus laevis and tropicalis is 76%. The homology of the immunogen o...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (prostate cell)
Loading amount 50 µg
Specification prostate cell
Gel Running Conditions Reduced Denaturing (10 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Dec 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human colon cancer tissue)
Specification human colon cancer tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
Username

Dr. Jaykumar Thumar

Verified customer

投稿 Dec 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Neural stem cells derived from human embryonic ste)
Specification Neural stem cells derived from human embryonic ste
Preparation Cell harvesting/tissue preparation method: Accutase digested single cell suspension of tissue culture cells
Sample buffer: PBS
Fixation BD Fixation/Permeabilization solution
Permeabilization Yes - BD Fixation/Permeabilization solution
Gating Strategy live cells
Username

Dr. Jennifer Moore

Verified customer

投稿 Sep 06 2010

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