リコンビナント
RabMAb

Anti-Musashi 1 / Msi1 抗体 [EP1302] (ab52865)

製品の概要

  • 製品名
    Anti-Musashi 1 / Msi1 antibody [EP1302]
    Musashi 1 / Msi1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
  • 特異性
    Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, IHC-Fr, WB, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Chicken, Human, Quail
  • 免疫原

    corresponding to Human Musashi 1/ Msi1 (N terminal).
    (Peptide available as ab178003)

  • ポジティブ・コントロール
    • PC12 cells, SH-SY-5Y cell lysate and human brain tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IHC-Fr 1/100.
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Musashi 1 / Msi1 peptide (ab178003).

For unpurified use at 1/2000.

Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/40.

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
    • 組織特異性
      Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
    • 配列類似性
      Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
    • ドメイン
      The first RNA recognition motif binds more strongly to RNA compared to the second one.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Msi 1 antibody
      • Msi1 antibody
      • MSI1H_HUMAN antibody
      • Musashi homolog 1 antibody
      • Musashi RNA binding protein 1 antibody
      • Musashi-1 antibody
      • Musashi1 antibody
      • RNA binding protein Musashi homolog 1 antibody
      • RNA-binding protein Musashi homolog 1 antibody
      see all

    画像

    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution (unpurified) + SH-SY-5Y cell lysate at 10 µg/ml

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
    • Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
    • Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized. The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.

      See Abreview

    参考文献

    This product has been referenced in:
    • Shou Z  et al. Overexpression of Musashi-1 protein is associated with progression and poor prognosis of gastric cancer. Oncol Lett 13:3556-3566 (2017). Read more (PubMed: 28521458) »
    • Lu X  et al. High-frequency repetitive transcranial magnetic stimulation for treating moderate traumatic brain injury in rats: A pilot study. Exp Ther Med 13:2247-2254 (2017). Read more (PubMed: 28565833) »

    See all 19 Publications for this product

    レビューと Q&A

    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 15 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (Teratoma)
    Specification
    Teratoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Feb 19 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica Bond Epitope Retrieval 2
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    投稿 Apr 02 2013

    Thank you very much for your interest our products.

    To our knowledge, neither ab21628 nor ab52865 have been tested in Xenopus. The homology of the immunogen of ab21628 with Xenopus laevis and tropicalis is 76%. The homology of the immunogen o...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 29 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (prostate cell)
    Loading amount
    50 µg
    Specification
    prostate cell
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 04 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Dr. Ruxandra Sirbulescu

    Verified customer

    投稿 Dec 08 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human colon cancer tissue)
    Specification
    human colon cancer tissue
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
    Username

    Dr. Jaykumar Thumar

    Verified customer

    投稿 Dec 31 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (Neural stem cells derived from human embryonic ste)
    Specification
    Neural stem cells derived from human embryonic ste
    Preparation
    Cell harvesting/tissue preparation method: Accutase digested single cell suspension of tissue culture cells
    Sample buffer: PBS
    Fixation
    BD Fixation/Permeabilization solution
    Permeabilization
    Yes - BD Fixation/Permeabilization solution
    Gating Strategy
    live cells
    Username

    Dr. Jennifer Moore

    Verified customer

    投稿 Sep 06 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse Brain)
    Loading amount
    20 µg
    Specification
    Mouse Brain
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 17 2010

    1-10 of 20 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録