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製品の概要

  • 製品名Anti-Mus81 antibody [MTA30 2G10/3]
    Mus81 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MTA30 2G10/3] to Mus81
  • アプリケーション適用あり: ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Mus81 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23144634
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 発生段階Expressed in S phase and G2 phase.
  • 細胞内局在Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Crossover junction endonuclease MUS81 antibody
      • FLJ21012 antibody
      • FLJ44872 antibody
      • Mus 81 antibody
      • MUS81 antibody
      • MUS81 Endonuclease Homolog antibody
      • MUS81_HUMAN antibody
      see all

    Anti-Mus81 antibody [MTA30 2G10/3] 画像

    • Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

      Predicted band size : 66 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    • Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) 使用論文

    This product has been referenced in:
    • Meisenberg C  et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res N/A:N/A (2016). WB ; Human . Read more (PubMed: 27789688) »
    • Thangavel S  et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol 208:545-62 (2015). Read more (PubMed: 25733713) »

    See all 20 Publications for this product

    ab14387 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab14387 の abreview を投稿する ab14387 に関する問い合わせをする

    10 Abreviews or Q&A

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    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application Western blot
    Loading amount 5 µg
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Human Cell lysate - whole cell (U2OS)
    Specification U2OS
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Dr. Remi Buisson

    Verified customer

    投稿 Oct 31 2014

    Immunoprecipitation abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 1000 µg
    Read More
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    Abcam user community

    Verified customer

    投稿 Jun 20 2014

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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    Abcam user community

    Verified customer

    投稿 Jun 10 2014

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
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    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount 30 µg
    Specification Mouse embryonic fibroblasts
    Gel Running Conditions Reduced Denaturing
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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    Abcam user community

    Verified customer

    投稿 Dec 13 2011

    Immunoprecipitation abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa cells)
    Total protein in input 200 µg
    Specification HeLa cells
    Immuno-precipitation step Protein A/G
    Read More
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    Abcam user community

    Verified customer

    投稿 Apr 06 2011

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 p53wt cells)
    Loading amount 30 µg
    Specification HCT116 p53wt cells
    Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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    Abcam user community

    Verified customer

    投稿 Nov 04 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Average
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (GM00637 human fibroblasts)
    Specification GM00637 human fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.4% Triton X-100
    Blocking step FCS as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
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    Abcam user community

    Verified customer

    投稿 Aug 31 2007

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Expression hMus81 in Baculovirus system)
    Loading amount 10000 cells
    Specification Expression hMus81 in Baculovirus system
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Dr. Olga Mazin

    Verified customer

    投稿 Feb 12 2007

    I am looking for an antibody to mouse Mus81. I know that your data sheet says that cross reactivity in species other than human has not yet been tested, but do you think your antibody will cross react with mouse Mus81? Thanks for you help.

    Thank you for your enquiry. Yes, ab14387 has only so far been tested for cross-reactivity with human Mus81 and we don't know how it will work with other species. I really can't say whether or not it will detect mouse Mus81. You might want to check to s...

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