Mus81 protein.
Our Abpromise guarantee covers the use of ab14387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use at an assay dependent concentration. PubMed: 23144634 | |
WB | 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. | |
IP | Use at an assay dependent concentration. | |
Flow Cyt | Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ab14387 staining Mus81 in GM00637 Human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% FCS for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour 30 minutes at 37°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody (1/600).
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