製品の概要

  • 製品名
    Anti-Mus81 antibody [MTA30 2G10/3]
    Mus81 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MTA30 2G10/3] to Mus81
  • アプリケーション
    適用あり: ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Mus81 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23144634
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 発生段階
    Expressed in S phase and G2 phase.
  • 細胞内局在
    Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Anti-Mus81 antibody [MTA30 2G10/3] 画像

  • Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) 使用論文

This product has been referenced in:
  • Meisenberg C  et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . Read more (PubMed: 28180300) »
  • Meisenberg C  et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res N/A:N/A (2016). WB ; Human . Read more (PubMed: 27789688) »

See all 22 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Treatment
cells were transfected with siMus81 siRNA
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 01 2017

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Remi Buisson

Verified customer

投稿 Oct 31 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
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投稿 Jun 20 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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投稿 Jun 10 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
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投稿 Nov 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
30 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Verified customer

投稿 Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
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投稿 Apr 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 p53wt cells)
Loading amount
30 µg
Specification
HCT116 p53wt cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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投稿 Nov 04 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (GM00637 human fibroblasts)
Specification
GM00637 human fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.4% Triton X-100
Blocking step
FCS as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

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投稿 Aug 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Expression hMus81 in Baculovirus system)
Loading amount
10000 cells
Specification
Expression hMus81 in Baculovirus system
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Olga Mazin

Verified customer

投稿 Feb 12 2007

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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