製品の概要

  • 製品名
    Anti-Mus81 antibody [MTA30 2G10/3]
  • 製品の詳細
    Mouse monoclonal [MTA30 2G10/3] to Mus81
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Mus81 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23144634
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 発生段階
    Expressed in S phase and G2 phase.
  • 細胞内局在
    Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Crossover junction endonuclease MUS81 antibody
      • FLJ21012 antibody
      • FLJ44872 antibody
      • Mus 81 antibody
      • MUS81 antibody
      • MUS81 Endonuclease Homolog antibody
      • MUS81_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

      Predicted band size: 66 kDa
      Observed band size: 64 kDa (why is the actual band size different from the predicted?)

    • Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunocytochemistry/ Immunofluorescence - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
      Immunocytochemistry/ Immunofluorescence - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)This image is courtesy of an anonymous Abreview

      ab14387 staining Mus81 in GM00637 Human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% FCS for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour 30 minutes at 37°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody (1/600).

      See Abreview

    参考文献

    This product has been referenced in:

    See all 27 Publications for this product

    ab14387 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    11 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (U2OS)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    cells were transfected with siMus81 siRNA
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Sep 01 2017

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Dr. Remi Buisson

    Verified customer

    投稿 Oct 31 2014

    Immunoprecipitation abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    1000 µg
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Jun 20 2014

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jun 10 2014

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    30 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Dec 13 2011

    Immunoprecipitation abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    200 µg
    Specification
    HeLa cells
    Immuno-precipitation step
    Protein A/G
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Apr 06 2011

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 p53wt cells)
    Loading amount
    30 µg
    Specification
    HCT116 p53wt cells
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 04 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (GM00637 human fibroblasts)
    Specification
    GM00637 human fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.4% Triton X-100
    Blocking step
    FCS as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Aug 31 2007

    Western blot abreview for Anti-Mus81 antibody [MTA30 2G10/3]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Expression hMus81 in Baculovirus system)
    Loading amount
    10000 cells
    Specification
    Expression hMus81 in Baculovirus system
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
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    Username

    Dr. Olga Mazin

    Verified customer

    投稿 Feb 12 2007

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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