製品の概要
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製品名Anti-Mus81 antibody [MTA30 2G10/3]
Mus81 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [MTA30 2G10/3] to Mus81
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アプリケーション適用あり: ICC/IF, WB, IP, Flow Cytmore details
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種交差性交差種: Human
非交差種: Mouse -
免疫原
Mus81 protein.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: PBS -
Concentration information loading... -
精製度Protein A purified
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特記事項（精製）from TCS
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ポリ/モノモノクローナル
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クローン名MTA30 2G10/3
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ミエローマSp2/0-Ag14
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アイソタイプIgG2a
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab14387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 23144634 | |
| WB | 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
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組織特異性Widely expressed.
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配列類似性Belongs to the XPF family.
Contains 1 ERCC4 domain. -
発生段階Expressed in S phase and G2 phase.
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細胞内局在Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
- Information by UniProt
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参照データベース
- Entrez Gene: 80198 Human
- Omim: 606591 Human
- SwissProt: Q96NY9 Human
- Unigene: 288798 Human
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別名
- Crossover junction endonuclease MUS81 antibody
- FLJ21012 antibody
- FLJ44872 antibody
see all
Anti-Mus81 antibody [MTA30 2G10/3] 画像
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![Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](http://a.abcam.com/ps/products/14/ab14387/Images/ab14387_1.jpg)
Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size : 66 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](http://a.abcam.com/ps/products/14/ab14387/Images/Mus81-Primary-antibodies-ab14387-4.jpg)
Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) 使用論文
This product has been referenced in:
- Meisenberg C et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . Read more (PubMed: 28180300) »
- Meisenberg C et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res N/A:N/A (2016). WB ; Human . Read more (PubMed: 27789688) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"