製品の概要

  • 製品名Anti-Mus81 antibody
    Mus81 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mus81
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 196 and 380 of Human Mus81 (NP_079404)

  • ポジティブ・コントロール
    • HepG2, NIH-3T3 and HeLa cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 発生段階Expressed in S phase and G2 phase.
  • 細胞内局在Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Anti-Mus81 antibody 画像

  • Anti-Mus81 antibody (ab97391) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Anti-Mus81 antibody (ab97391) at 1/500 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97391 at 1/100 dilution (Panel 1) and merged with DNA probe (Panel 2).

Anti-Mus81 antibody (ab97391) 使用論文

ab97391 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Abcam user community

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投稿 Jul 14 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
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Abcam user community

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投稿 Jun 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 30 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Abcam user community

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投稿 May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Abcam user community

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投稿 Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"