製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MURF2
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    HIFSFSWLNSLNE

    , corresponding to C terminal amino acids 536-546 of Human MURF2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/0.1 - 1/0.3. Detects a band of approximately 85 kDa (predicted molecular weight: 60 kDa). PubMed: 17272810
IHC-Fr Use at an assay dependent dilution.

ターゲット情報

  • 機能
    May regulate gene expression and protein turnover in muscle cells.
  • 組織特異性
    Highly expressed in muscle. Low-level expression in liver.
  • 配列類似性
    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • 細胞内局在
    Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MURF 2 antibody
    • MURF-2 antibody
    • MuRF2 antibody
    • Muscle specific ring finger 2 antibody
    • Muscle specific RING finger protein 2 antibody
    • Muscle-specific RING finger protein 2 antibody
    • RING finger protein 29 antibody
    • RNF 29 antibody
    • RNF29 antibody
    • TRI55_HUMAN antibody
    • TRIM 55 antibody
    • Trim55 antibody
    • Tripartite motif containing 55 antibody
    • Tripartite motif containing protein 55 antibody
    • Tripartite motif-containing protein 55 antibody
    see all

Anti-MURF2 antibody 画像

  • Anti-MURF2 antibody (ab4387) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Predicted band size : 60 kDa

Anti-MURF2 antibody (ab4387) 使用論文

This product has been referenced in:
  • He J  et al. MuRF2 regulates PPAR?1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol 14:97 (2015). WB . Read more (PubMed: 26242235) »
  • Cullup T  et al. Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy. Nat Genet 45:83-7 (2013). Read more (PubMed: 23222957) »

See all 5 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Muscle cell)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
60 µg
Specification
Muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Joao de Oliveira Silvestre

Verified customer

投稿 Feb 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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