製品の概要

  • 製品名Anti-MURF1 antibody
    MURF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MURF1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MURF1.

    (Peptide available as ab87125.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: human skeletal muscle; mouse skeletal muscle; mouse heart.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • 組織特異性Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • 発生段階Expressed throughout all developmental stages.
  • ドメインThe RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • 細胞内局在Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

Anti-MURF1 antibody 画像

  • All lanes : Anti-MURF1 antibody (ab77577) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 110 kDa,30 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab77577 staining in Mouse Skeletal Muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77577, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MURF1 antibody (ab77577) 使用論文

This product has been referenced in:
  • Sun B  et al. The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Mol Med Rep 14:3113-21 (2016). Read more (PubMed: 27511050) »
  • Padrão AI  et al. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia. Arch Biochem Biophys 567:13-21 (2015). Read more (PubMed: 25575785) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (High fat diet-fed SD rat skeletal muscle)
Loading amount 30 µg
Specification High fat diet-fed SD rat skeletal muscle
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 16 2013

Thank you for your reply. I have checked our catalog and we do have a positive control available, human skeletal muscle whole cell lysate ab29330. We do not have samples available, but if the antibodies produced many non-specific bands in WB, this i...

Read More

Thank you for contacting Abcam regarding out FBX32 and MURF1 antibodies. I would like to recommend two products that will be suitable for your experiments. Regarding Anti-Fbx32, I would like to recommend ab92281. As shown on the online datasheet, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"