製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MURC
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 95-180 (KVSAHIKDVK ARVEKQQIHV KKVEVKQEEI MKKNKFRVVI FQEKFRCPTS LSVVKDRNLT ENQEEDDDDI FDPPVDLSSD EEYYVE) of Human MURC.

  • ポジティブ・コントロール
    • Human skeletal muscle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway.
  • 配列類似性
    Belongs to the PTRF/SDPR family.
  • 細胞内局在
    Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • murC antibody
    • MURC_HUMAN antibody
    • Muscle-related coiled-coil protein antibody
    • Muscle-restricted coiled-coil protein antibody

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121647, at 1/1000 dilution, staining MURC in paraffin-embedded Human skeletal muscle tissue, by Immunohistochemistry.

参考文献

ab121647 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.1% Triton-x 100
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Feb 16 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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