製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Mupp1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Platypus, Elephant
  • 免疫原

    A region within amino acids 1125-1150 of Human Mupp1 (NP_003820.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 219 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性
    MUPP1 was first identified as a protein interacting with type 2C serotonin receptor. It acts as a scaffolding protein at tight junctions where it has been reported to interact with integral proteins, anchoring them to the F-actin cytoskeleten. It is also thought to be important in the osmotic stress response in kidney cells and has been shown to play a role in promoting G protein coupling to receptors.
  • 細胞内局在
    Plasma membrane
  • 参照データベース
  • 別名
    • MPDZ antibody
    • Multi PDZ domain protein 1 antibody
    • Multi-PDZ domain protein 1 antibody
    • multiple PDZ domain protein antibody

画像

  • All lanes : Anti-Mupp1 antibody (ab101277) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 219 kDa


    Exposure time : 3 minutes
  • Detection of Mupp1 by Western Blot of Immunprecipitate.
    ab101277, at 1 µg/ml, staining Mupp1 in HeLa whole cell lysate immunoprecipitated using ab101277 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.

参考文献

ab101277 has not yet been referenced specifically in any publications.

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