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RabMAb

Anti-Munc 13-4 抗体 [EPR4914] (ab109113)

製品の概要

  • 製品名
    Anti-Munc 13-4 antibody [EPR4914]
    Munc 13-4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4914] to Munc 13-4
  • アプリケーション
    適用あり: WB, ICC, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide from a region of human Munc 13-4 (Q70J99).

  • ポジティブ・コントロール
    • A673, K562, HepG2, Molt-4, and RAW264.7.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4914
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
    • 組織特異性
      Expressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
    • 関連疾患
      Defects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • 配列類似性
      Belongs to the unc-13 family.
      Contains 2 C2 domains.
      Contains 1 MHD1 (MUNC13 homology domain 1) domain.
      Contains 1 MHD2 (MUNC13 homology domain 2) domain.
    • ドメイン
      The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
    • 細胞内局在
      Cytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      There are 3 isoforms produced by alternative splicing.
    • 別名
      • FHL 3 antibody
      • FHL3 antibody
      • FLJ00067 antibody
      • HLH 3 antibody
      • HLH3 antibody
      • HPLH 3 antibody
      • HPLH3 antibody
      • Jinx antibody
      • Munc13 4 antibody
      • Munc13-4 antibody
      • Protein unc 13 homolog D antibody
      • Protein unc-13 homolog D antibody
      • UN13D_HUMAN antibody
      • Unc 13 homolog D antibody
      • UNC 13D antibody
      • Unc-13 homolog D (C. elegans) antibody
      • Unc13 homolog D (C elegans) antibody
      • Unc13 homolog D antibody
      • UNC13, C. elegans, homolog of, D antibody
      • UNC13D antibody
      • Unc13h4 antibody
      see all

    画像

    • All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Molt-4 cell lysate
      Lane 5 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 123 kDa
    • Flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.

    参考文献

    ab109113 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for contacting us regarding Ab109113.

    The Flow Cytometry data shown on our webpage usedthe following secondary antibody from JacksonImmuno Research:

    Code Number: 111-095-144 Description:
    Fluorescein (FITC) AffiniPu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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