Anti-Munc 13-4 抗体 [EPR4914] (ab109113)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4914] to Munc 13-4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Munc 13-4 antibody [EPR4914]
Munc 13-4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4914] to Munc 13-4 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A673, K562, HepG2, Molt-4, and RAW264.7. Flow Cyt (intra): Molt-4.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4914 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
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特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa. |
ターゲット情報
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機能
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells. -
組織特異性
Expressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. -
関連疾患
Defects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
配列類似性
Belongs to the unc-13 family.
Contains 2 C2 domains.
Contains 1 MHD1 (MUNC13 homology domain 1) domain.
Contains 1 MHD2 (MUNC13 homology domain 2) domain. -
ドメイン
The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome. -
細胞内局在
Cytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles. - Information by UniProt
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参照データベース
- Entrez Gene: 201294 Human
- Entrez Gene: 70450 Mouse
- Omim: 608897 Human
- SwissProt: Q70J99 Human
- SwissProt: B2RUP2 Mouse
- Unigene: 41045 Human
- Unigene: 259460 Mouse
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製品の状態
There are 3 isoforms produced by alternative splicing. -
別名
- FHL 3 antibody
- FHL3 antibody
- FLJ00067 antibody
see all
画像
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All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
Intracellular flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab109113 は 3 報の論文で使用されています。
- Takushi SE et al. Lentiviral Gene Therapy for Familial Hemophagocytic Lymphohistiocytosis Type 3, Caused by UNC13D Genetic Defects. Hum Gene Ther 31:626-638 (2020). PubMed: 32253931
- Krawczynski K et al. Unc-13 homolog D mediates an antiviral effect of the chromosome 19 microRNA cluster miR-517a. J Cell Sci 134:N/A (2020). PubMed: 33093239
- Xu R et al. Munc13-4 mediates human neutrophil elastase-induced airway mucin5AC hypersecretion by interacting with syntaxin2. Mol Med Rep 18:1015-1024 (2018). PubMed: 29767240