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ab106071 is a human B cell marker panel containing CD5 mouse monoclonal (PE-conjugated), CD19 mouse monoclonal (APC-conjugated), CD20 mouse monoclonal (FITC-conjugated) and CD45 mouse monoclonal (PE/Cy7-conjugated) antibodies, tested for use as a cocktail in flow cytometry.
The human B cell marker panel contains a selection of the most popular B cell associated CD markers, conjugated to a wide spectrum of fluorochromes for optimal detection using flow cytometry. Matched isotype controls are available (ab91363, ab91358, ab81197, ab103534).
|ab1157 - Anti-CD5 antibody [CRIS1] (Phycoerythrin)||1 x 50 tests|
|ab1169 - Anti-CD20 antibody [B9E9] (FITC)||1 x 50 tests|
|ab18224 - Anti-CD19 antibody [LT19] (Allophycocyanin)||1 x 50 tests|
|ab46729 - Anti-CD45 antibody [F10-89-4] (PE/Cy7 ®)||1 x 50 tests|
Our Abpromise guarantee covers the use of ab106071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. Typical usage: 2.5-5µl of each antibody for 106 cells. Optimal volumes should be determined by the end user.|
ab106071 staining of B cells in whole human blood by flow cytometry.
Peripheral blood was collected into Li Heparin LH/9 ml monovettes. Red blood cells were lysed with lysis buffer (for 5 ml: 41.45 g NH4Cl, 5 g NaHCO3, 1 ml 0.5M EDTA in H2O) for 20 min at room temperature. After spinning down, the obtained cell pellet was washed twice with PBS. The cells were stained with the antibodies (2.5 – 5 µl of each antibody/1x106 cells in 100 µl) for 20 min at room temperature in the dark. Samples were washed twice with cell sorting buffer (3 % FCS in PBS) and resuspended. Flow cytometry was performed on a FACSCalibur (Becton Dickinson) at 488 nm (FL-1, 2, 3) and 633 nm (FL-4), and analysed using Flojo software.
ab106071 has not yet been referenced specifically in any publications.