Multicolor Human B cell marker panel (CD5-PE, CD19-APC, CD20-FITC and CD45-PE/Cy7®) (ab106071)

製品の概要

  • 製品名
    Multicolor Human B cell marker panel (CD5-PE, CD19-APC, CD20-FITC and CD45-PE/Cy7®)
  • 製品の概要

    ab106071 is a human B cell marker panel containing CD5 mouse monoclonal (PE-conjugated), CD19 mouse monoclonal (APC-conjugated), CD20 mouse monoclonal (FITC-conjugated) and CD45 mouse monoclonal (PE/Cy7-conjugated) antibodies, tested for use as a cocktail in flow cytometry.

    The human B cell marker panel contains a selection of the most popular B cell associated CD markers, conjugated to a wide spectrum of fluorochromes for optimal detection using flow cytometry. Matched isotype controls are available (ab91363, ab91358, ab81197, ab103534).

  • アプリケーション
    適用あり: Flow Cytmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. Typical usage: 2.5-5µl of each antibody for 106 cells. Optimal volumes should be determined by the end user.

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  • ab106071 staining of B cells in whole human blood by flow cytometry.

    Peripheral blood was collected into Li Heparin LH/9 ml monovettes. Red blood cells were lysed with lysis buffer (for 5 ml: 41.45 g NH4Cl, 5 g NaHCO3, 1 ml 0.5M EDTA in H2O) for 20 min at room temperature. After spinning down, the obtained cell pellet was washed twice with PBS. The cells were stained with the antibodies (2.5 – 5 µl of each antibody/1x106 cells in 100 µl) for 20 min at room temperature in the dark. Samples were washed twice with cell sorting buffer (3 % FCS in PBS) and resuspended. Flow cytometry was performed on a FACSCalibur (Becton Dickinson) at 488 nm (FL-1, 2, 3) and 633 nm (FL-4), and analysed using Flojo software.

    Matched isotype control antibodies were used under the same conditions (ab81197, ab91363

参考文献

ab106071 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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