製品の概要

  • 製品名Anti-MUC5B antibody
    MUC5B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MUC5B
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 5650 - 5750 of Human MUC5B.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human normal colon FFPE section. IF: HepG2 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
  • 組織特異性Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
  • 配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 3 HAT repeats.
    Contains 3 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • ドメインThe cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
  • 翻訳後修飾Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2300002I04Rik antibody
    • A130042M24 antibody
    • AV085033 antibody
    • Cervical mucin antibody
    • Cervical mucin MUC5B antibody
    • High molecular weight salivary mucin MG1 antibody
    • MG1 antibody
    • MUC-5B antibody
    • MUC5 antibody
    • MUC5B antibody
    • MUC5B_HUMAN antibody
    • MUC9 antibody
    • Mucin 5 subtype B, tracheobronchial antibody
    • Mucin 5B antibody
    • Mucin 5B oligomeric mucus/gel forming antibody
    • Mucin-5 subtype B antibody
    • Mucin-5B antibody
    • Sublingual gland mucin antibody
    • tracheobronchial antibody
    see all

Anti-MUC5B antibody 画像

  • IHC image of MUC5B staining in Human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab87376 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87376, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MUC5B antibody (ab87376) 使用論文

ab87376 has not yet been referenced specifically in any publications.

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