製品の概要

  • 製品名Anti-MUC4 antibody
    MUC4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to MUC4
  • アプリケーション適用あり: ELISA, WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment with tag: GVSLFPYGAD AGDLEFVRRT VDFTSPLFKP ATGFPLGSSL RDSLYFTDNG QIIFPESDYQ IFSYPNPLPT GFTGRDPVAL VAPFWDDADF STGRGTTFYQ EYETFYGEHS , corresponding to amino acids 79-189 of Human MUC4

  • ポジティブ・コントロール
    • HeLa cell lysate (WB). Human stomach (IHC-P).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged MUC4 is approximately 0.3ng/ml as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 235 kDa).
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
  • 組織特異性Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
  • 配列類似性Contains 1 AMOP domain.
    Contains 2 EGF-like domains.
    Contains 1 NIDO domain.
    Contains 1 VWFD domain.
  • 発生段階Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.
  • 翻訳後修飾Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
    mucrnin-4 alpha chain is highly O-glycosylated.
    mucin-4 beta chain is predominantly N-glycosylated.
  • 細胞内局在Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ascites sialoglycoprotein 1 antibody
    • Ascites sialoglycoprotein 2 antibody
    • Ascites sialoglycoprotein antibody
    • ASGP antibody
    • ASGP-1 antibody
    • ASGP-2 antibody
    • HSA276359 antibody
    • MUC 4 antibody
    • MUC-4 antibody
    • Muc4 antibody
    • MUC4_HUMAN antibody
    • Mucin 4 antibody
    • Mucin 4 cell surface associated antibody
    • Mucin 4 tracheobronchial antibody
    • Mucin-4 beta chain antibody
    • Pancreatic adenocarcinoma mucin antibody
    • Testis mucin antibody
    • Tracheobronchial mucin antibody
    • Tracheobronchial mucin Fragment antibody
    see all

Anti-MUC4 antibody 画像

  • Anti-MUC4 antibody (ab60720) at 1 µg/ml + HeLa cell lysate at 25 µg

    Predicted band size : 235 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • MUC4 antibody (ab60720) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.

Anti-MUC4 antibody (ab60720) 使用論文

This product has been referenced in:
  • Tang J  et al. The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer. J Exp Clin Cancer Res 35:91 (2016). WB ; Human . Read more (PubMed: 27287498) »
  • Lee H  et al. DA-6034-induced mucin secretion via Ca2+-dependent pathways through P2Y receptor stimulation. Invest Ophthalmol Vis Sci 55:6565-74 (2014). WB ; Human . Read more (PubMed: 25212776) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (oviductal tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization No
Specification oviductal tissue
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Sep 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Serum (mouse serum)
Loading amount 30 µg
Specification mouse serum
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 May 25 2012

Thank you for your patience.
The immunogen has now been added to our catalog as ab130768.
http://www.abcam.com/index.html?datasheet=130768
This is the immunogen (protein fragment) that was used to raise antibody ab60720.
If you woul...

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Thank you for contacting us.
The lab let me know that the immunogen would be available for sale. I do not have yet any information about catalog number, amount and price, but will let you know as soon as I do.
I hope this is good news for you...

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Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"