製品の概要

  • 製品名Anti-MUC4 antibody [8G-7]
    MUC4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8G-7] to MUC4
  • アプリケーション適用あり: IP, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    非交差種: Rat
  • 免疫原

    MUC4

  • ポジティブ・コントロール
    • Lung cancer
  • 特記事項


    Total Protein Concentration: ~10 mg/ml.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 14729877
ICC/IF Use at an assay dependent concentration. PubMed: 14729877
WB Use at an assay dependent concentration. PubMed: 14729877
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
  • 組織特異性Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
  • 配列類似性Contains 1 AMOP domain.
    Contains 2 EGF-like domains.
    Contains 1 NIDO domain.
    Contains 1 VWFD domain.
  • 発生段階Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.
  • 翻訳後修飾Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
    mucrnin-4 alpha chain is highly O-glycosylated.
    mucin-4 beta chain is predominantly N-glycosylated.
  • 細胞内局在Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ascites sialoglycoprotein 1 antibody
    • Ascites sialoglycoprotein 2 antibody
    • Ascites sialoglycoprotein antibody
    • ASGP antibody
    • ASGP-1 antibody
    • ASGP-2 antibody
    • HSA276359 antibody
    • MUC 4 antibody
    • MUC-4 antibody
    • Muc4 antibody
    • MUC4_HUMAN antibody
    • Mucin 4 antibody
    • Mucin 4 cell surface associated antibody
    • Mucin 4 tracheobronchial antibody
    • Mucin-4 beta chain antibody
    • Pancreatic adenocarcinoma mucin antibody
    • Testis mucin antibody
    • Tracheobronchial mucin antibody
    • Tracheobronchial mucin Fragment antibody
    see all

Anti-MUC4 antibody [8G-7] 画像

  • IHC image of ab52263 staining in human pancreatic carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52263, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MUC4 antibody [8G-7] (ab52263) 使用論文

This product has been referenced in:
  • Zhi X  et al. MUC4-induced nuclear translocation of ß-catenin: A novel mechanism for growth, metastasis and angiogenesis in pancreatic cancer. Cancer Lett 346:104-13 (2014). Human . Read more (PubMed: 24374017) »
  • Möller E  et al. FUS-CREB3L2/L1-Positive Sarcomas Show a Specific Gene Expression Profile with Upregulation of CD24 and FOXL1. Clin Cancer Res 17:2646-56 (2011). IHC-P ; Human . Read more (PubMed: 21536545) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Human Tissue sections (urinary tract)
Specification urinary tract
Permeabilization No
Fixative Formaldehyde
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Verified customer

投稿 May 24 2013

Thank you for your reply.


I can confirm that the buffer is: PBS pH 7.3 with 10% BSA and 0.9% sodium azide.


If you were interested in conjugating this antibody yourself, I would recommend the following products:


Read More

Thank you for contacting Abcam regarding ab52263.


I can confirm that this antibody does target an extracellular region of the MUC4 protein.


Regarding the request for biotinylation, this may be possible but will require a bul...

Read More

Thank you for contacting us. This MUC4 antibody (ab52263) recognizes an extracellular epitope. I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"