製品の概要

  • 製品名Anti-MUC2 antibody [B306.1]
    MUC2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B306.1] to MUC2
  • 特異性This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic MUC2 tandem repeat consensus sequence.

  • ポジティブ・コントロール
    • Colon carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • 組織特異性Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • 配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • 翻訳後修飾O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • 細胞内局在Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Anti-MUC2 antibody [B306.1] 画像

  • Ab7848 staining human normal colon. Staining is localised to the membrane.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-MUC2 antibody [B306.1] (ab7848) 使用論文

This product has been referenced in:
  • Fishbein TM  et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). IHC-Fr ; Human . Read more (PubMed: 19519821) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (GI)
Loading amount 50 µg
Specification GI
Gel Running Conditions Reduced Denaturing
Blocking step proprietary as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Sep 27 2007

Thank you for contacting us with your enquiry. The SwissProt database links on the datasheets for these two antibodies give some information on the tissue expression of Muc1 and muc2. Muc 2 (ab7848) is expressed in Colon, small intestine, colon...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"