Anti-MUC2 抗体 (ab97386)
Key features and details
- Rabbit polyclonal to MUC2
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MUC2 antibody
MUC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MUC2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Common marmoset -
免疫原
Synthetic peptide within Human MUC2 (C terminal). The exact sequence is proprietary. Carrier protein conjugated.
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ポジティブ・コントロール
- IHC-P: Mouse colon tissue. Mouse and human intestine tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97386の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (6) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
組織特異性
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
翻訳後修飾
O-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
細胞内局在
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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参照データベース
- Entrez Gene: 4583 Human
- Entrez Gene: 17831 Mouse
- Omim: 158370 Human
- SwissProt: Q02817 Human
- SwissProt: Q80Z19 Mouse
- Unigene: 315 Human
- Unigene: 2041 Mouse
- Unigene: 461296 Mouse
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別名
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
画像
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Paraffin-embedded mouse colon tissue stained for MUC2 using ab97386 at a 1/2000 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Paraffin-embedded mouse intestine tissue stained for MUC2 using ab97386 at a 1/2000 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Paraffin-embedded human intestine tissue stained for MUC2 using ab97386 at a 1/750 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab97386 は 5 報の論文で使用されています。
- Kim KS et al. Vertical sleeve gastrectomy induces enteroendocrine cell differentiation of intestinal stem cells through bile acid signaling. JCI Insight 7:N/A (2022). PubMed: 35503251
- Guo L et al. Dietary Goji Shapes the Gut Microbiota to Prevent the Liver Injury Induced by Acute Alcohol Intake. Front Nutr 9:929776 (2022). PubMed: 35898713
- Tian M et al. Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota. Nutrients 13:N/A (2021). PubMed: 33807544
- Prados ME et al. Betulinic acid hydroxamate prevents colonic inflammation and fibrosis in murine models of inflammatory bowel disease. Acta Pharmacol Sin 42:1124-1138 (2021). PubMed: 32811965
- Chiu K et al. The Impact of Di-Isononyl Phthalate Exposure on Specialized Epithelial Cells in the Colon. Toxicol Sci 184:142-153 (2021). PubMed: 34453847