The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100. Using streptavidin-Biotin system or polymer system. High temperature antigen retrieval is recommended (boiling tissue in 10 mM citrate buffer for 10 minutes) this enhances the signal and may require further dilution of the antibody.
IHC-Fr: 1/50 - 1/100.
This antibody stains normal colon and colonic carcinomas where it produces intense perinuclear staining in goblet cells. It also reacts with normal and neoplastic breast tissues and with prostate carcinoma.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
O-glycosylated. May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
Secreted. In the intestine, secreted into the inner and outer mucus layers.
ab76774 at 1/100 dilution, staining MUC2 in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The DAB staining procedure was used with hematoxylin as counterstain.
Xu R et al. Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Exp Ther Med11:943-950 (2016).
Read more (PubMed: 26998017) »
Luo Q et al. Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells. Infect Immun82:509-21 (2014).
Read more (PubMed: 24478067) »