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Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163
We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Our Abpromise guarantee covers the use of ab11197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20418870|
|Flow Cyt||Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. This product does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 621.5 kDa.|
ab11197 staining MUC2 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with PBS + 0.2% Triton X-100 and blocked with 10% serum for 30 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 2 hours at 37°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"