製品の概要

  • 製品名
    Anti-MUC16 antibody [X325]
    MUC16 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [X325] to MUC16
  • 特異性
    Epitope specificity group B (ISOBM classification).
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified from human ovarian carcinoma).

  • 特記事項
    Concentration varies from lot to lot and can be provided on request.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
IHC-P
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of >1000 kDa.


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • 組織特異性
      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • 配列類似性
      Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • ドメイン
      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • 翻訳後修飾
      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • 細胞内局在
      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    画像

    • ab10033 staining MUC16 in Human lung tissue (alveolocytes type II) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human benign ovarian tumor tissue (serous papillary cystadenoma) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human bladder carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    参考文献

    This product has been referenced in:
    • Lee H  et al. DA-6034-induced mucin secretion via Ca2+-dependent pathways through P2Y receptor stimulation. Invest Ophthalmol Vis Sci 55:6565-74 (2014). Read more (PubMed: 25212776) »
    • Shimizu A  et al. Coexpression of MUC16 and mesothelin is related to the invasion process in pancreatic ductal adenocarcinoma. Cancer Sci 103:739-46 (2012). Human . Read more (PubMed: 22320398) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus Monkey Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Dako protein block as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Sheng Zhong

    Verified customer

    投稿 Nov 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (urinary tract)
    Specification
    urinary tract
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 24 2013

    Thank you for contacting us.

    Following Western blot conditions have been tested with our anti-CA-125 MAbs:

    1. CA125 is immobilized on the nitrocellulose membrane for 30 min (400 mA, 25 mM Tris-Hcl, 192 mM Glycin, 0,1% SDS, 20% ethano...

    Read More

    Thank you very much for contacting us.

    I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

    I am v...

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    Thank you for your resposne.

    As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

    As EL...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (human colon and pancreas)
    Total protein in input
    5000 µg
    Specification
    human colon and pancreas
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    投稿 Aug 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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