製品の概要

  • 製品名Anti-MUC16 antibody [OC125]
    MUC16 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OC125] to MUC16
  • 特異性Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
  • アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.

  • エピトープThis antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
  • ポジティブ・コントロール
    • Ovarian cancer or endocervix.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method.
IHC-Fr 1/50 - 1/100. ABC Method.

ターゲット情報

  • 機能Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • 組織特異性Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • 配列類似性Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • ドメインComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • 翻訳後修飾Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • 細胞内局在Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Anti-MUC16 antibody [OC125] 画像

  • Overlay histogram showing HeLa cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MUC16 antibody [OC125] (ab693) 使用論文

This product has been referenced in:
  • Zhang J  et al. Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice. PLoS One 9:e104388 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25118887) »
  • Athanassiadou P  et al. Expression of cathepsin-D, CA125 and epidermal growth factor receptor in imprint smears of ovarian carcinoma. Gynecol Obstet Invest 43:125-30 (1997). Read more (PubMed: 9067721) »

See all 8 Publications for this product

Product Wall

Thank you for your interest in the MUC16 antibody, clone OC125. The papers from 1981 and 1983 that are listed in the references section of the ab693 datasheet describe production and characterization of the antibody. The antigen was not a peptide; it w...

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The IgG concentration of the lot you have is 25.5 ug/mL. The antibody is not conjugated, so there is no F/P ratio.

Thank you for your reply. The concentration for the current lot is 50.5 ug/mL. Please let me know if you have any further questions.

Thank you for contacting us. The concentration is batch dependent. Have you already purchased the product? If so I can provide the concentration if you can send the lot number. Thank you!

I am very pleased to hear you would like to accept our offer and test ab693 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Addition...

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Thank you for your reply and for clarifying the questions.

I can confirm that both these antibodies (ab693 and ab134093) are tested and covered by our 6 month guarantee for flow cytometry.

However, I am sorry they have not been tr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"