Anti-MUC13 抗体 (ab65109)

製品の概要

  • 製品名Anti-MUC13 antibody
    MUC13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MUC13
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) from an internal sequence.

  • ポジティブ・コントロール
    • WB: Extracts from COLO, 293 and HeLa cells. IHC-P: Human colon carcinoma tissue. IF: HepG2 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
  • 組織特異性Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
  • 配列類似性Contains 3 EGF-like domains.
    Contains 1 SEA domain.
  • 翻訳後修飾Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
    Highly N-glycosylated.
  • 細胞内局在Cell membrane. Secreted. Also exists as a soluble form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Down-regulated in colon cancer 1 antibody
    • DRCC1 antibody
    • FLJ20063 antibody
    • MUC 13 antibody
    • MUC-13 antibody
    • Muc13 antibody
    • MUC13 Down regulated in colon cancer 1 antibody
    • MUC13_HUMAN antibody
    • Mucin 13, cell surface associated antibody
    • Mucin-13 antibody
    • PRO20221 antibody
    • RECC antibody
    • UNQ6194 antibody
    see all

Anti-MUC13 antibody 画像

  • All lanes : Anti-MUC13 antibody (ab65109) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from 293 cells
    Lane 3 : extracts from HeLa cells
    Lane 4 : extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ICC/IF image of ab65109 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MUC13 antibody (ab65109) 使用論文

ab65109 has not yet been referenced specifically in any publications.

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Thank you for contacting us and your interest in our products.

As for ab124654 the immunogen sequence used to raise the anti-MUC13 antibody (ab65109) is proprietary information. However, in order to aid your research we will sometimes make an ...

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Application Western blot
Sample Mouse Serum (serum)
Loading amount 30 µg
Specification serum
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Jun 20 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas and pancreatic tumor)
Specification pancreas and pancreatic tumor
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

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投稿 Jun 20 2012

Thank you for contacting Abcam.

The immunogen sequence for ab65109is from ********* of the human protein sequence.

If there is anything else I can help you with, please let me know.

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and be...

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Unfortunately we don't have 50µg unit of ab65109, only 100µg. We carry over 70,000 products, it isn't feasible for us to keep different sample sizes of our products.

Thank you for contacting us. Unfortunately we do not have any monoclonal MUC13 antibody in our catalog. The alternative product we have for the detection of MUC13 is a rabbit polyclonal antibody, reference ab72050, www.abcam.com/ab72050 . ...

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Thank you for contacting us. The immunogen sequence of ab65109 is part of the transmembrane and the cytoplasmic regions of the Human MUC13. I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"